The Effect Of Ph On Enzyme Activity

The Effect of pH on Function of Catecholase Enzyme in spud Abstract In biochemical reactions, enzymes activity could be transmitd found on these factors: parsimoniousness of subst dictates, salt concentration, al loseeric regulators, temperature and pH. Inventing the hypothesis is change magnitude concentration of pH would annex the activity of enzyme Catecholase and the rate of reaction. Therefore, the purpose of this experiment was to test the frame of pH on Catecholase (one kind of enzymes in potato) activity by using pH12 buffer. By using the Spectronic 20D+ Spectrophotometer, the change in absorbance everyplace time give the gate be recorded. The result proved: trend to the hypothesis, at pH 12, activity of enzyme Catecholase and the rate of reaction decreased. Introduction pH is a measure of the concentration of atomic number 1 H+ in resolving power so the higher concentration hydrogen ion, the frown pH. pH is one of the most important fa ctors affect to human body. pH was divided three parts on pH subdue from pH 0 to pH 14: at pH 7, solutions are neutral. pH > 7 is alkalinity purlieu that pass on declare hydrogen ion, and pH < 7 is acidic environment that go out donate hydrogen ion. On the other hand, increasing hydrogen ion concentration will reduce the bound surface cation concentration.

decrease hydrogen ion concentration will increase the hydroxyl stalk ion concentration (M. Chaplin, 2004). Enzymes are one of the most efficient catalysts in biochemical reactions. They are used to speed up the reaction rates by providing an alterna tive reaction pathway of turn down activat! ion energy, which is the minimum energy that requires for reactions occur. Catalysts take part in a reaction (chemical involved), but they do not allow for any changes in chemical reaction. (Farabee, 2001) Enzymes are proteins made of a combination of different amino acids. Therefore, the enzymes may be preoccupied their function when structure of protein is changed under conditions such as cofactors, coenzymes,...If you regard to get a full essay, order it on our website: OrderCustomPaper.com

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